Studies of Developing Human Hair Shaft Cells In Vitro
نویسندگان
چکیده
منابع مشابه
Genotype analysis using human hair shaft.
Analysis of genotypes for genetic predisposition of diseases has profound and widespread clinical application and has become a fundamental tool in the study of molecular epidemiology. In this study, we report a convenient and completely noninvasive approach to perform genotyping using terminal cephalic hair shaft. The average and median DNA amounts purified from the hair shaft are 112 +/- 180 (...
متن کاملTowards expansion of human hair follicle stem cells in vitro.
OBJECTIVES Multipotential human hair follicle stem cells can differentiate into various cell lineages and thus are investigated here as potential autologous sources for regenerative medicine. Towards this end, we have attempted to expand these cells, directly isolated from minimal amounts of hair follicle explants, to numbers more suitable for stem-cell therapy. MATERIALS AND METHODS Two type...
متن کاملHuman hair growth in vitro.
We report for the first time the successful maintenance and growth of human hair follicles in vitro. Human anagen hair follicles were isolated by microdissection from human scalp skin. Isolation of the hair follicles was achieved by cutting the follicle at the dermo-subcutaneous fat interface using a scalpel blade. Intact hair follicles were then removed from the fat using watchmakers' forceps....
متن کاملIsolation and Quantification of Glycosaminoglycans from Human Hair Shaft
BACKGROUND There is evidence that glycosaminoglycans (GAGs) are present in the hair shaft within the follicle but there are no studies regarding GAGs isolation and measurement in the human hair shaft over the scalp surface, it means, in the free hair shaft. OBJECTIVE The purpose of our research was to isolate and measure the total GAGs from human free hair shaft. METHODS Seventy-five health...
متن کاملEstablishment of human hair follicle mesenchymal stem cells with overexpressed human hepatocyte growth factor
Objective(s): Chronic liver disease has become a major health problem that causes serious damage to human health. Since the existing treatment effect was not ideal, we need to seek new treatment methods. Materials and Methods: We utilized the gene recombination technology to obtain the human hair mesenchymal stem cells which overexpression of human hepatocyte growth factor (hHGF). Furthermore, ...
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 1988
ISSN: 0022-202X
DOI: 10.1111/1523-1747.ep12462566